by Winex » Wed Jul 28, 2010 11:46 pm
by MoapaPk » Thu Jul 29, 2010 12:20 am
mrchad9 wrote:booo! Could have at least left the frustration gif!!!!
by lcarreau » Thu Jul 29, 2010 1:01 am
peladoboton wrote:jschrock wrote:All I use (for energy/hydration) if I'm not just drinking water...
by Charles » Thu Jul 29, 2010 2:36 pm
James_W wrote:Ahh moran is the mega-cybernet version.
I think Llama steaks are too ugly for anyone to eat.
by MoapaPk » Thu Jul 29, 2010 3:09 pm
KristoriaBlack wrote:That EnragedTroll seemed like a smart cookie. I hope he didn't pick up on this already. Seems like you guys had quite the chitter-chatter there. Too bad I missed it. I kinda liked that Troll... he gave me a good tip with the Sigma antibodies.
by Bob Sihler » Thu Jul 29, 2010 3:25 pm
MikeTX wrote:TheOrglingLlama wrote:
my god, she's so hot - even just sitting there eating popcorn!
by dskoon » Thu Jul 29, 2010 3:40 pm
Bob Sihler wrote:MikeTX wrote:TheOrglingLlama wrote:
my god, she's so hot - even just sitting there eating popcorn!
Better get to her fast, though; after a few hours sitting there munching on popcorn and reading this stuff, she may not be so hot anymore.
by Charles » Thu Jul 29, 2010 3:57 pm
Bob Sihler wrote:MikeTX wrote:TheOrglingLlama wrote:
my god, she's so hot - even just sitting there eating popcorn!
Better get to her fast, though; after a few hours sitting there munching on popcorn and reading this stuff, she may not be so hot anymore.
by Bob Sihler » Thu Jul 29, 2010 4:51 pm
WeakFemale wrote:Psst, Kristoria, here is another antibody tip from toxo. Hopefully Lawyerfag won't delete this before you see it:
For Westerns, dilute the primary antibody in plain TBST without milk or BSA. As long as you have blocked beforehand there is no problem with noise. That way, the same working dilution of primary antibody can be used over and over again, up to a month or so (You could add a bit of azide for longer storage, which would be washed off by the time for HRP detection).
Many people don't realize you can do this and discard antibodies diluted in milk/TBST after one use since milk doesn't keep. I save bundles on antibodies this way.
(About the weird handle: I was going to troll the "Dragging SOs to mountains" thread using it, but realized I don't have the necessary jpg)
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